sequence-specific primer (ssp) typing kit Search Results


campylobacter species dna  (ATCC)
95
ATCC campylobacter species dna
Flow diagram of the experiments conducted in each objective. (A) In the first objective, an internal control (IC) and primers for the genus <t>Campylobacter,</t> C. coli, C. jejuni, C. fetus, and C. hyointestinalis were developed. The presentation of two taxon names in the same box (on the right) represents multiplex reactions. Boxes with thick walls represent nested PCR. Names in the box labeled “Test taxa” represent taxa that were used to determine the specificities of PCRs; numbers in parentheses following the taxon names represent the numbers of isolates tested. (B) In the second objective, bovine feces were inoculated with various concentrations of C. coli, C. fetus, C. hyointestinalis, and C. jejuni (target densities in log10 CFU per gram are presented in circles); the control treatment (“0”) was not inoculated with campylobacters. CFUs were enumerated in the inoculated and control feces by using dilution plating. Concurrently, <t>DNA</t> was extracted from all fecal treatments and subjected to PCR with the primers shown in objective A, and amplicons were electrophoresed. (C) In the third objective, uninoculated bovine feces were collected from eight dairy cows (C1 to C8). Campylobacters were isolated and enumerated on four media maintained at three temperatures. Representative colonies were selected, established in pure cultures, and identified by using physiological characters, PCR with primers for specific taxa and, if required, extraction of DNA and sequencing of a portion of the 16S rRNA gene. During the course of this experiment, C. lanienae was frequently isolated on Karmali medium at 40°C. As a result, a nested primer set was developed for this taxon, and the C. lanienae primers were used in a multiplex PCR with primers for C. fetus. DNA was extracted from fecal samples obtained from the eight cows and subjected to PCR, and amplicons were electrophoresed. The experiments in the second and third objectives were conducted three times on separate occasions.
Campylobacter Species Dna, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antisense primer  (Thermo Fisher)
98
Thermo Fisher antisense primer
Flow diagram of the experiments conducted in each objective. (A) In the first objective, an internal control (IC) and primers for the genus <t>Campylobacter,</t> C. coli, C. jejuni, C. fetus, and C. hyointestinalis were developed. The presentation of two taxon names in the same box (on the right) represents multiplex reactions. Boxes with thick walls represent nested PCR. Names in the box labeled “Test taxa” represent taxa that were used to determine the specificities of PCRs; numbers in parentheses following the taxon names represent the numbers of isolates tested. (B) In the second objective, bovine feces were inoculated with various concentrations of C. coli, C. fetus, C. hyointestinalis, and C. jejuni (target densities in log10 CFU per gram are presented in circles); the control treatment (“0”) was not inoculated with campylobacters. CFUs were enumerated in the inoculated and control feces by using dilution plating. Concurrently, <t>DNA</t> was extracted from all fecal treatments and subjected to PCR with the primers shown in objective A, and amplicons were electrophoresed. (C) In the third objective, uninoculated bovine feces were collected from eight dairy cows (C1 to C8). Campylobacters were isolated and enumerated on four media maintained at three temperatures. Representative colonies were selected, established in pure cultures, and identified by using physiological characters, PCR with primers for specific taxa and, if required, extraction of DNA and sequencing of a portion of the 16S rRNA gene. During the course of this experiment, C. lanienae was frequently isolated on Karmali medium at 40°C. As a result, a nested primer set was developed for this taxon, and the C. lanienae primers were used in a multiplex PCR with primers for C. fetus. DNA was extracted from fecal samples obtained from the eight cows and subjected to PCR, and amplicons were electrophoresed. The experiments in the second and third objectives were conducted three times on separate occasions.
Antisense Primer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcr ssp  (Thermo Fisher)
95
Thermo Fisher pcr ssp
Flow diagram of the experiments conducted in each objective. (A) In the first objective, an internal control (IC) and primers for the genus <t>Campylobacter,</t> C. coli, C. jejuni, C. fetus, and C. hyointestinalis were developed. The presentation of two taxon names in the same box (on the right) represents multiplex reactions. Boxes with thick walls represent nested PCR. Names in the box labeled “Test taxa” represent taxa that were used to determine the specificities of PCRs; numbers in parentheses following the taxon names represent the numbers of isolates tested. (B) In the second objective, bovine feces were inoculated with various concentrations of C. coli, C. fetus, C. hyointestinalis, and C. jejuni (target densities in log10 CFU per gram are presented in circles); the control treatment (“0”) was not inoculated with campylobacters. CFUs were enumerated in the inoculated and control feces by using dilution plating. Concurrently, <t>DNA</t> was extracted from all fecal treatments and subjected to PCR with the primers shown in objective A, and amplicons were electrophoresed. (C) In the third objective, uninoculated bovine feces were collected from eight dairy cows (C1 to C8). Campylobacters were isolated and enumerated on four media maintained at three temperatures. Representative colonies were selected, established in pure cultures, and identified by using physiological characters, PCR with primers for specific taxa and, if required, extraction of DNA and sequencing of a portion of the 16S rRNA gene. During the course of this experiment, C. lanienae was frequently isolated on Karmali medium at 40°C. As a result, a nested primer set was developed for this taxon, and the C. lanienae primers were used in a multiplex PCR with primers for C. fetus. DNA was extracted from fecal samples obtained from the eight cows and subjected to PCR, and amplicons were electrophoresed. The experiments in the second and third objectives were conducted three times on separate occasions.
Pcr Ssp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcr-sequence specific primers high resolution methods  (ClinImmune Labs)
90
ClinImmune Labs pcr-sequence specific primers high resolution methods
Flow diagram of the experiments conducted in each objective. (A) In the first objective, an internal control (IC) and primers for the genus <t>Campylobacter,</t> C. coli, C. jejuni, C. fetus, and C. hyointestinalis were developed. The presentation of two taxon names in the same box (on the right) represents multiplex reactions. Boxes with thick walls represent nested PCR. Names in the box labeled “Test taxa” represent taxa that were used to determine the specificities of PCRs; numbers in parentheses following the taxon names represent the numbers of isolates tested. (B) In the second objective, bovine feces were inoculated with various concentrations of C. coli, C. fetus, C. hyointestinalis, and C. jejuni (target densities in log10 CFU per gram are presented in circles); the control treatment (“0”) was not inoculated with campylobacters. CFUs were enumerated in the inoculated and control feces by using dilution plating. Concurrently, <t>DNA</t> was extracted from all fecal treatments and subjected to PCR with the primers shown in objective A, and amplicons were electrophoresed. (C) In the third objective, uninoculated bovine feces were collected from eight dairy cows (C1 to C8). Campylobacters were isolated and enumerated on four media maintained at three temperatures. Representative colonies were selected, established in pure cultures, and identified by using physiological characters, PCR with primers for specific taxa and, if required, extraction of DNA and sequencing of a portion of the 16S rRNA gene. During the course of this experiment, C. lanienae was frequently isolated on Karmali medium at 40°C. As a result, a nested primer set was developed for this taxon, and the C. lanienae primers were used in a multiplex PCR with primers for C. fetus. DNA was extracted from fecal samples obtained from the eight cows and subjected to PCR, and amplicons were electrophoresed. The experiments in the second and third objectives were conducted three times on separate occasions.
Pcr Sequence Specific Primers High Resolution Methods, supplied by ClinImmune Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcr-ssp (sequence specific primer) kit  (GenoVision VertriebsgesmbH)
90
GenoVision VertriebsgesmbH pcr-ssp (sequence specific primer) kit
Flow diagram of the experiments conducted in each objective. (A) In the first objective, an internal control (IC) and primers for the genus <t>Campylobacter,</t> C. coli, C. jejuni, C. fetus, and C. hyointestinalis were developed. The presentation of two taxon names in the same box (on the right) represents multiplex reactions. Boxes with thick walls represent nested PCR. Names in the box labeled “Test taxa” represent taxa that were used to determine the specificities of PCRs; numbers in parentheses following the taxon names represent the numbers of isolates tested. (B) In the second objective, bovine feces were inoculated with various concentrations of C. coli, C. fetus, C. hyointestinalis, and C. jejuni (target densities in log10 CFU per gram are presented in circles); the control treatment (“0”) was not inoculated with campylobacters. CFUs were enumerated in the inoculated and control feces by using dilution plating. Concurrently, <t>DNA</t> was extracted from all fecal treatments and subjected to PCR with the primers shown in objective A, and amplicons were electrophoresed. (C) In the third objective, uninoculated bovine feces were collected from eight dairy cows (C1 to C8). Campylobacters were isolated and enumerated on four media maintained at three temperatures. Representative colonies were selected, established in pure cultures, and identified by using physiological characters, PCR with primers for specific taxa and, if required, extraction of DNA and sequencing of a portion of the 16S rRNA gene. During the course of this experiment, C. lanienae was frequently isolated on Karmali medium at 40°C. As a result, a nested primer set was developed for this taxon, and the C. lanienae primers were used in a multiplex PCR with primers for C. fetus. DNA was extracted from fecal samples obtained from the eight cows and subjected to PCR, and amplicons were electrophoresed. The experiments in the second and third objectives were conducted three times on separate occasions.
Pcr Ssp (Sequence Specific Primer) Kit, supplied by GenoVision VertriebsgesmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
pcr-ssp (sequence specific primer) kit - by Bioz Stars, 2026-05
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nested polymerase chain reaction  (Feto Maternal and GenetYX Center)
90
Feto Maternal and GenetYX Center nested polymerase chain reaction
Flow diagram of the experiments conducted in each objective. (A) In the first objective, an internal control (IC) and primers for the genus <t>Campylobacter,</t> C. coli, C. jejuni, C. fetus, and C. hyointestinalis were developed. The presentation of two taxon names in the same box (on the right) represents multiplex reactions. Boxes with thick walls represent nested PCR. Names in the box labeled “Test taxa” represent taxa that were used to determine the specificities of PCRs; numbers in parentheses following the taxon names represent the numbers of isolates tested. (B) In the second objective, bovine feces were inoculated with various concentrations of C. coli, C. fetus, C. hyointestinalis, and C. jejuni (target densities in log10 CFU per gram are presented in circles); the control treatment (“0”) was not inoculated with campylobacters. CFUs were enumerated in the inoculated and control feces by using dilution plating. Concurrently, <t>DNA</t> was extracted from all fecal treatments and subjected to PCR with the primers shown in objective A, and amplicons were electrophoresed. (C) In the third objective, uninoculated bovine feces were collected from eight dairy cows (C1 to C8). Campylobacters were isolated and enumerated on four media maintained at three temperatures. Representative colonies were selected, established in pure cultures, and identified by using physiological characters, PCR with primers for specific taxa and, if required, extraction of DNA and sequencing of a portion of the 16S rRNA gene. During the course of this experiment, C. lanienae was frequently isolated on Karmali medium at 40°C. As a result, a nested primer set was developed for this taxon, and the C. lanienae primers were used in a multiplex PCR with primers for C. fetus. DNA was extracted from fecal samples obtained from the eight cows and subjected to PCR, and amplicons were electrophoresed. The experiments in the second and third objectives were conducted three times on separate occasions.
Nested Polymerase Chain Reaction, supplied by Feto Maternal and GenetYX Center, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ssp micro ssp dna typing kit  (Thermo Fisher)
99
Thermo Fisher ssp micro ssp dna typing kit
Flow diagram of the experiments conducted in each objective. (A) In the first objective, an internal control (IC) and primers for the genus <t>Campylobacter,</t> C. coli, C. jejuni, C. fetus, and C. hyointestinalis were developed. The presentation of two taxon names in the same box (on the right) represents multiplex reactions. Boxes with thick walls represent nested PCR. Names in the box labeled “Test taxa” represent taxa that were used to determine the specificities of PCRs; numbers in parentheses following the taxon names represent the numbers of isolates tested. (B) In the second objective, bovine feces were inoculated with various concentrations of C. coli, C. fetus, C. hyointestinalis, and C. jejuni (target densities in log10 CFU per gram are presented in circles); the control treatment (“0”) was not inoculated with campylobacters. CFUs were enumerated in the inoculated and control feces by using dilution plating. Concurrently, <t>DNA</t> was extracted from all fecal treatments and subjected to PCR with the primers shown in objective A, and amplicons were electrophoresed. (C) In the third objective, uninoculated bovine feces were collected from eight dairy cows (C1 to C8). Campylobacters were isolated and enumerated on four media maintained at three temperatures. Representative colonies were selected, established in pure cultures, and identified by using physiological characters, PCR with primers for specific taxa and, if required, extraction of DNA and sequencing of a portion of the 16S rRNA gene. During the course of this experiment, C. lanienae was frequently isolated on Karmali medium at 40°C. As a result, a nested primer set was developed for this taxon, and the C. lanienae primers were used in a multiplex PCR with primers for C. fetus. DNA was extracted from fecal samples obtained from the eight cows and subjected to PCR, and amplicons were electrophoresed. The experiments in the second and third objectives were conducted three times on separate occasions.
Ssp Micro Ssp Dna Typing Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcr/sequence-specific primer (ssp) kit  (BioSewoom Inc)
90
BioSewoom Inc pcr/sequence-specific primer (ssp) kit
Flow diagram of the experiments conducted in each objective. (A) In the first objective, an internal control (IC) and primers for the genus <t>Campylobacter,</t> C. coli, C. jejuni, C. fetus, and C. hyointestinalis were developed. The presentation of two taxon names in the same box (on the right) represents multiplex reactions. Boxes with thick walls represent nested PCR. Names in the box labeled “Test taxa” represent taxa that were used to determine the specificities of PCRs; numbers in parentheses following the taxon names represent the numbers of isolates tested. (B) In the second objective, bovine feces were inoculated with various concentrations of C. coli, C. fetus, C. hyointestinalis, and C. jejuni (target densities in log10 CFU per gram are presented in circles); the control treatment (“0”) was not inoculated with campylobacters. CFUs were enumerated in the inoculated and control feces by using dilution plating. Concurrently, <t>DNA</t> was extracted from all fecal treatments and subjected to PCR with the primers shown in objective A, and amplicons were electrophoresed. (C) In the third objective, uninoculated bovine feces were collected from eight dairy cows (C1 to C8). Campylobacters were isolated and enumerated on four media maintained at three temperatures. Representative colonies were selected, established in pure cultures, and identified by using physiological characters, PCR with primers for specific taxa and, if required, extraction of DNA and sequencing of a portion of the 16S rRNA gene. During the course of this experiment, C. lanienae was frequently isolated on Karmali medium at 40°C. As a result, a nested primer set was developed for this taxon, and the C. lanienae primers were used in a multiplex PCR with primers for C. fetus. DNA was extracted from fecal samples obtained from the eight cows and subjected to PCR, and amplicons were electrophoresed. The experiments in the second and third objectives were conducted three times on separate occasions.
Pcr/Sequence Specific Primer (Ssp) Kit, supplied by BioSewoom Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcr/sequence-specific primer (ssp) kit/product/BioSewoom Inc
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nalm6 atcc cvcl 0092 hek293t cells atcc crl 11268 oligonucleotides itr specific qpcr forward primer  (ATCC)
99
ATCC nalm6 atcc cvcl 0092 hek293t cells atcc crl 11268 oligonucleotides itr specific qpcr forward primer
Flow diagram of the experiments conducted in each objective. (A) In the first objective, an internal control (IC) and primers for the genus <t>Campylobacter,</t> C. coli, C. jejuni, C. fetus, and C. hyointestinalis were developed. The presentation of two taxon names in the same box (on the right) represents multiplex reactions. Boxes with thick walls represent nested PCR. Names in the box labeled “Test taxa” represent taxa that were used to determine the specificities of PCRs; numbers in parentheses following the taxon names represent the numbers of isolates tested. (B) In the second objective, bovine feces were inoculated with various concentrations of C. coli, C. fetus, C. hyointestinalis, and C. jejuni (target densities in log10 CFU per gram are presented in circles); the control treatment (“0”) was not inoculated with campylobacters. CFUs were enumerated in the inoculated and control feces by using dilution plating. Concurrently, <t>DNA</t> was extracted from all fecal treatments and subjected to PCR with the primers shown in objective A, and amplicons were electrophoresed. (C) In the third objective, uninoculated bovine feces were collected from eight dairy cows (C1 to C8). Campylobacters were isolated and enumerated on four media maintained at three temperatures. Representative colonies were selected, established in pure cultures, and identified by using physiological characters, PCR with primers for specific taxa and, if required, extraction of DNA and sequencing of a portion of the 16S rRNA gene. During the course of this experiment, C. lanienae was frequently isolated on Karmali medium at 40°C. As a result, a nested primer set was developed for this taxon, and the C. lanienae primers were used in a multiplex PCR with primers for C. fetus. DNA was extracted from fecal samples obtained from the eight cows and subjected to PCR, and amplicons were electrophoresed. The experiments in the second and third objectives were conducted three times on separate occasions.
Nalm6 Atcc Cvcl 0092 Hek293t Cells Atcc Crl 11268 Oligonucleotides Itr Specific Qpcr Forward Primer, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene exp lgals8 mm01332239 m1  (Thermo Fisher)
91
Thermo Fisher gene exp lgals8 mm01332239 m1
Galectin-8 Is Expressed in Lymphoid Tissues (A) qRT-PCR analysis of Galectin-8 ( <t>Lgals8</t> ) mRNA levels in kidney, bone marrow (BM), spleen, lymph nodes (LNs), and liver of C56BL/6 WT mice. Values were normalized with respect to the BM condition for each mouse. n = 5 mice pooled from N = 3 independent experiments. Bar graphs indicate mean ± SEM. (B) Representative images of H&E staining (left) and β-galactosidase staining (right) of LN cryosection from heterozygous mouse bearing a LacZ expression cassette on one allele of the Lgals8 locus. Arrowheads on the inset highlight β-galactosidase staining within the SCS area. Scale bar, 150 μm. (C) Representative images of serial lymph node cryosections stained for β-galactosidase (Galectin-8) and macrophages (Mac1) or B cells (B220). Scale bar, 200 μm. Zooms highlight the spatial localization of Galectin-8 together with macrophages and B cells at the SCS. Scale bar, 30 μm. See also <xref ref-type=Figure S1 . " width="250" height="auto" />
Gene Exp Lgals8 Mm01332239 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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caption a7 species strain s b species specific primer pcr results c bilon biinf biden bigal b adolescentis atcc 15703 t  (ATCC)
96
ATCC caption a7 species strain s b species specific primer pcr results c bilon biinf biden bigal b adolescentis atcc 15703 t
Bacterial strains and results of PCR assays in which species-specific primers BiLON, BiINF, BiDEN, and BiGAL were used a
Caption A7 Species Strain S B Species Specific Primer Pcr Results C Bilon Biinf Biden Bigal B Adolescentis Atcc 15703 T, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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caption a7 species strain s b species specific primer pcr results c bilon biinf biden bigal b adolescentis atcc 15703 t - by Bioz Stars, 2026-05
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gene exp cxadr mm00438355 m1  (Thermo Fisher)
98
Thermo Fisher gene exp cxadr mm00438355 m1
Bacterial strains and results of PCR assays in which species-specific primers BiLON, BiINF, BiDEN, and BiGAL were used a
Gene Exp Cxadr Mm00438355 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Flow diagram of the experiments conducted in each objective. (A) In the first objective, an internal control (IC) and primers for the genus Campylobacter, C. coli, C. jejuni, C. fetus, and C. hyointestinalis were developed. The presentation of two taxon names in the same box (on the right) represents multiplex reactions. Boxes with thick walls represent nested PCR. Names in the box labeled “Test taxa” represent taxa that were used to determine the specificities of PCRs; numbers in parentheses following the taxon names represent the numbers of isolates tested. (B) In the second objective, bovine feces were inoculated with various concentrations of C. coli, C. fetus, C. hyointestinalis, and C. jejuni (target densities in log10 CFU per gram are presented in circles); the control treatment (“0”) was not inoculated with campylobacters. CFUs were enumerated in the inoculated and control feces by using dilution plating. Concurrently, DNA was extracted from all fecal treatments and subjected to PCR with the primers shown in objective A, and amplicons were electrophoresed. (C) In the third objective, uninoculated bovine feces were collected from eight dairy cows (C1 to C8). Campylobacters were isolated and enumerated on four media maintained at three temperatures. Representative colonies were selected, established in pure cultures, and identified by using physiological characters, PCR with primers for specific taxa and, if required, extraction of DNA and sequencing of a portion of the 16S rRNA gene. During the course of this experiment, C. lanienae was frequently isolated on Karmali medium at 40°C. As a result, a nested primer set was developed for this taxon, and the C. lanienae primers were used in a multiplex PCR with primers for C. fetus. DNA was extracted from fecal samples obtained from the eight cows and subjected to PCR, and amplicons were electrophoresed. The experiments in the second and third objectives were conducted three times on separate occasions.

Journal:

Article Title: Use of PCR for Direct Detection of Campylobacter Species in Bovine Feces †

doi: 10.1128/AEM.69.6.3435-3447.2003

Figure Lengend Snippet: Flow diagram of the experiments conducted in each objective. (A) In the first objective, an internal control (IC) and primers for the genus Campylobacter, C. coli, C. jejuni, C. fetus, and C. hyointestinalis were developed. The presentation of two taxon names in the same box (on the right) represents multiplex reactions. Boxes with thick walls represent nested PCR. Names in the box labeled “Test taxa” represent taxa that were used to determine the specificities of PCRs; numbers in parentheses following the taxon names represent the numbers of isolates tested. (B) In the second objective, bovine feces were inoculated with various concentrations of C. coli, C. fetus, C. hyointestinalis, and C. jejuni (target densities in log10 CFU per gram are presented in circles); the control treatment (“0”) was not inoculated with campylobacters. CFUs were enumerated in the inoculated and control feces by using dilution plating. Concurrently, DNA was extracted from all fecal treatments and subjected to PCR with the primers shown in objective A, and amplicons were electrophoresed. (C) In the third objective, uninoculated bovine feces were collected from eight dairy cows (C1 to C8). Campylobacters were isolated and enumerated on four media maintained at three temperatures. Representative colonies were selected, established in pure cultures, and identified by using physiological characters, PCR with primers for specific taxa and, if required, extraction of DNA and sequencing of a portion of the 16S rRNA gene. During the course of this experiment, C. lanienae was frequently isolated on Karmali medium at 40°C. As a result, a nested primer set was developed for this taxon, and the C. lanienae primers were used in a multiplex PCR with primers for C. fetus. DNA was extracted from fecal samples obtained from the eight cows and subjected to PCR, and amplicons were electrophoresed. The experiments in the second and third objectives were conducted three times on separate occasions.

Article Snippet: Primer sequences for the amplification of Campylobacter species DNA from bovine feces The following reference strains were used to test the primers that we developed: Arcobacter butzleri (ATCC 49616 [American Type Culture Collection]), A. cryaerophilus (ATCC 49942), C. coli (HC 1111 [Health Canada] and ATCC 49941), C. concisus (ATCC 33237), C. fetus subsp. fetus (ATCC 25936), C. fetus subsp. venerealis (ATCC 19438), C. hyointestinalis subsp. hyointestinalis (ATCC 35217), C. hyointestinalis subsp. lawsonii (NCTC 12901 [National Collection of Type Cultures and Pathogenic Fungi]), C. jejuni (HC 1102, HC 1104, HC 1115, HC 1108, ATCC 29428, ATCC 49943, and ATCC 33291), C. lanienae (NCTC 13004), C. lari (ATCC 35221), and C. upsaliensis (LCDC 5424 [Laboratory Centre for Disease Control]).

Techniques: Multiplex Assay, Nested PCR, Labeling, Isolation, Sequencing

Primer sequences for the amplification of Campylobacter species DNA from bovine feces

Journal:

Article Title: Use of PCR for Direct Detection of Campylobacter Species in Bovine Feces †

doi: 10.1128/AEM.69.6.3435-3447.2003

Figure Lengend Snippet: Primer sequences for the amplification of Campylobacter species DNA from bovine feces

Article Snippet: Primer sequences for the amplification of Campylobacter species DNA from bovine feces The following reference strains were used to test the primers that we developed: Arcobacter butzleri (ATCC 49616 [American Type Culture Collection]), A. cryaerophilus (ATCC 49942), C. coli (HC 1111 [Health Canada] and ATCC 49941), C. concisus (ATCC 33237), C. fetus subsp. fetus (ATCC 25936), C. fetus subsp. venerealis (ATCC 19438), C. hyointestinalis subsp. hyointestinalis (ATCC 35217), C. hyointestinalis subsp. lawsonii (NCTC 12901 [National Collection of Type Cultures and Pathogenic Fungi]), C. jejuni (HC 1102, HC 1104, HC 1115, HC 1108, ATCC 29428, ATCC 49943, and ATCC 33291), C. lanienae (NCTC 13004), C. lari (ATCC 35221), and C. upsaliensis (LCDC 5424 [Laboratory Centre for Disease Control]).

Techniques: Amplification, Sequencing, Modification, Multiplex Assay

Primer sequences used to identify Campylobacter species isolated from bovine feces

Journal:

Article Title: Use of PCR for Direct Detection of Campylobacter Species in Bovine Feces †

doi: 10.1128/AEM.69.6.3435-3447.2003

Figure Lengend Snippet: Primer sequences used to identify Campylobacter species isolated from bovine feces

Article Snippet: Primer sequences for the amplification of Campylobacter species DNA from bovine feces The following reference strains were used to test the primers that we developed: Arcobacter butzleri (ATCC 49616 [American Type Culture Collection]), A. cryaerophilus (ATCC 49942), C. coli (HC 1111 [Health Canada] and ATCC 49941), C. concisus (ATCC 33237), C. fetus subsp. fetus (ATCC 25936), C. fetus subsp. venerealis (ATCC 19438), C. hyointestinalis subsp. hyointestinalis (ATCC 35217), C. hyointestinalis subsp. lawsonii (NCTC 12901 [National Collection of Type Cultures and Pathogenic Fungi]), C. jejuni (HC 1102, HC 1104, HC 1115, HC 1108, ATCC 29428, ATCC 49943, and ATCC 33291), C. lanienae (NCTC 13004), C. lari (ATCC 35221), and C. upsaliensis (LCDC 5424 [Laboratory Centre for Disease Control]).

Techniques: Isolation, Sequencing

Multiplex PCR for detection of Campylobacter species in bovine feces. Lane 1, 100-bp molecular weight marker (the dark band was at 500 bp); lane 2, DNA from uninoculated feces amplified with the Campylobacter genus-specific primer set; lane 3, DNA from uninoculated feces amplified with the Campylobacter genus-specific primer set (note the weak genus amplicon and the internal control amplicon at 465 bp); lane 4, DNA from uninoculated feces amplified with the C. jejuni-C. coli multiplex nested primer set (note the C. jejuni amplicon at 413 bp); lane 5, DNA from uninoculated feces amplified with the C. fetus-C. lanienae nested primer set (note the C. lanienae amplicon at 360 bp); lane 6, DNA from feces inoculated with C. coli ATCC 49941 at a density of ≈103 CFU g−1 and amplified with the C. jejuni-C. coli multiplex nested primer set (note the C. coli amplicon at 330 bp); lane 7, DNA from feces inoculated with C. hyointestinalis subsp. hyointestinalis ATCC 35217 at a density of ≈102 CFU g−1 and amplified with the C. hyointestinalis seminested primer set (note the C. hyointestinalis amplicon at 468 bp); lane 8, DNA from feces inoculated with C. fetus ATCC 25936 at a density of ≈102 CFU g−1 and amplified with the C. fetus-C. lanienae nested primer set (note the C. fetus amplicon at 473 bp); lane 9, negative control for the internal control and Campylobacter genus-specific primers (no template added); lane 10, negative control for C. jejuni-C. coli multiplex primers; lane 11, negative control for C. fetus-C. lanienae multiplex primers; lane 12, negative control for C. hyointestinalis subsp. hyointestinalis primers.

Journal:

Article Title: Use of PCR for Direct Detection of Campylobacter Species in Bovine Feces †

doi: 10.1128/AEM.69.6.3435-3447.2003

Figure Lengend Snippet: Multiplex PCR for detection of Campylobacter species in bovine feces. Lane 1, 100-bp molecular weight marker (the dark band was at 500 bp); lane 2, DNA from uninoculated feces amplified with the Campylobacter genus-specific primer set; lane 3, DNA from uninoculated feces amplified with the Campylobacter genus-specific primer set (note the weak genus amplicon and the internal control amplicon at 465 bp); lane 4, DNA from uninoculated feces amplified with the C. jejuni-C. coli multiplex nested primer set (note the C. jejuni amplicon at 413 bp); lane 5, DNA from uninoculated feces amplified with the C. fetus-C. lanienae nested primer set (note the C. lanienae amplicon at 360 bp); lane 6, DNA from feces inoculated with C. coli ATCC 49941 at a density of ≈103 CFU g−1 and amplified with the C. jejuni-C. coli multiplex nested primer set (note the C. coli amplicon at 330 bp); lane 7, DNA from feces inoculated with C. hyointestinalis subsp. hyointestinalis ATCC 35217 at a density of ≈102 CFU g−1 and amplified with the C. hyointestinalis seminested primer set (note the C. hyointestinalis amplicon at 468 bp); lane 8, DNA from feces inoculated with C. fetus ATCC 25936 at a density of ≈102 CFU g−1 and amplified with the C. fetus-C. lanienae nested primer set (note the C. fetus amplicon at 473 bp); lane 9, negative control for the internal control and Campylobacter genus-specific primers (no template added); lane 10, negative control for C. jejuni-C. coli multiplex primers; lane 11, negative control for C. fetus-C. lanienae multiplex primers; lane 12, negative control for C. hyointestinalis subsp. hyointestinalis primers.

Article Snippet: Primer sequences for the amplification of Campylobacter species DNA from bovine feces The following reference strains were used to test the primers that we developed: Arcobacter butzleri (ATCC 49616 [American Type Culture Collection]), A. cryaerophilus (ATCC 49942), C. coli (HC 1111 [Health Canada] and ATCC 49941), C. concisus (ATCC 33237), C. fetus subsp. fetus (ATCC 25936), C. fetus subsp. venerealis (ATCC 19438), C. hyointestinalis subsp. hyointestinalis (ATCC 35217), C. hyointestinalis subsp. lawsonii (NCTC 12901 [National Collection of Type Cultures and Pathogenic Fungi]), C. jejuni (HC 1102, HC 1104, HC 1115, HC 1108, ATCC 29428, ATCC 49943, and ATCC 33291), C. lanienae (NCTC 13004), C. lari (ATCC 35221), and C. upsaliensis (LCDC 5424 [Laboratory Centre for Disease Control]).

Techniques: Multiplex Assay, Molecular Weight, Marker, Amplification, Negative Control

Microbiological isolation and direct PCR detection of Campylobacter populations in inoculated bovine feces

Journal:

Article Title: Use of PCR for Direct Detection of Campylobacter Species in Bovine Feces †

doi: 10.1128/AEM.69.6.3435-3447.2003

Figure Lengend Snippet: Microbiological isolation and direct PCR detection of Campylobacter populations in inoculated bovine feces

Article Snippet: Primer sequences for the amplification of Campylobacter species DNA from bovine feces The following reference strains were used to test the primers that we developed: Arcobacter butzleri (ATCC 49616 [American Type Culture Collection]), A. cryaerophilus (ATCC 49942), C. coli (HC 1111 [Health Canada] and ATCC 49941), C. concisus (ATCC 33237), C. fetus subsp. fetus (ATCC 25936), C. fetus subsp. venerealis (ATCC 19438), C. hyointestinalis subsp. hyointestinalis (ATCC 35217), C. hyointestinalis subsp. lawsonii (NCTC 12901 [National Collection of Type Cultures and Pathogenic Fungi]), C. jejuni (HC 1102, HC 1104, HC 1115, HC 1108, ATCC 29428, ATCC 49943, and ATCC 33291), C. lanienae (NCTC 13004), C. lari (ATCC 35221), and C. upsaliensis (LCDC 5424 [Laboratory Centre for Disease Control]).

Techniques: Isolation

Isolation of campylobacters from bovine feces a

Journal:

Article Title: Use of PCR for Direct Detection of Campylobacter Species in Bovine Feces †

doi: 10.1128/AEM.69.6.3435-3447.2003

Figure Lengend Snippet: Isolation of campylobacters from bovine feces a

Article Snippet: Primer sequences for the amplification of Campylobacter species DNA from bovine feces The following reference strains were used to test the primers that we developed: Arcobacter butzleri (ATCC 49616 [American Type Culture Collection]), A. cryaerophilus (ATCC 49942), C. coli (HC 1111 [Health Canada] and ATCC 49941), C. concisus (ATCC 33237), C. fetus subsp. fetus (ATCC 25936), C. fetus subsp. venerealis (ATCC 19438), C. hyointestinalis subsp. hyointestinalis (ATCC 35217), C. hyointestinalis subsp. lawsonii (NCTC 12901 [National Collection of Type Cultures and Pathogenic Fungi]), C. jejuni (HC 1102, HC 1104, HC 1115, HC 1108, ATCC 29428, ATCC 49943, and ATCC 33291), C. lanienae (NCTC 13004), C. lari (ATCC 35221), and C. upsaliensis (LCDC 5424 [Laboratory Centre for Disease Control]).

Techniques: Isolation

Results of physiological and molecular typing of arbitrarily selected isolates of Arcobacter and Campylobacter recovered from bovine feces

Journal:

Article Title: Use of PCR for Direct Detection of Campylobacter Species in Bovine Feces †

doi: 10.1128/AEM.69.6.3435-3447.2003

Figure Lengend Snippet: Results of physiological and molecular typing of arbitrarily selected isolates of Arcobacter and Campylobacter recovered from bovine feces

Article Snippet: Primer sequences for the amplification of Campylobacter species DNA from bovine feces The following reference strains were used to test the primers that we developed: Arcobacter butzleri (ATCC 49616 [American Type Culture Collection]), A. cryaerophilus (ATCC 49942), C. coli (HC 1111 [Health Canada] and ATCC 49941), C. concisus (ATCC 33237), C. fetus subsp. fetus (ATCC 25936), C. fetus subsp. venerealis (ATCC 19438), C. hyointestinalis subsp. hyointestinalis (ATCC 35217), C. hyointestinalis subsp. lawsonii (NCTC 12901 [National Collection of Type Cultures and Pathogenic Fungi]), C. jejuni (HC 1102, HC 1104, HC 1115, HC 1108, ATCC 29428, ATCC 49943, and ATCC 33291), C. lanienae (NCTC 13004), C. lari (ATCC 35221), and C. upsaliensis (LCDC 5424 [Laboratory Centre for Disease Control]).

Techniques:

Microbiological isolation and PCR detection of campylobacters in bovine feces by replicate a

Journal:

Article Title: Use of PCR for Direct Detection of Campylobacter Species in Bovine Feces †

doi: 10.1128/AEM.69.6.3435-3447.2003

Figure Lengend Snippet: Microbiological isolation and PCR detection of campylobacters in bovine feces by replicate a

Article Snippet: Primer sequences for the amplification of Campylobacter species DNA from bovine feces The following reference strains were used to test the primers that we developed: Arcobacter butzleri (ATCC 49616 [American Type Culture Collection]), A. cryaerophilus (ATCC 49942), C. coli (HC 1111 [Health Canada] and ATCC 49941), C. concisus (ATCC 33237), C. fetus subsp. fetus (ATCC 25936), C. fetus subsp. venerealis (ATCC 19438), C. hyointestinalis subsp. hyointestinalis (ATCC 35217), C. hyointestinalis subsp. lawsonii (NCTC 12901 [National Collection of Type Cultures and Pathogenic Fungi]), C. jejuni (HC 1102, HC 1104, HC 1115, HC 1108, ATCC 29428, ATCC 49943, and ATCC 33291), C. lanienae (NCTC 13004), C. lari (ATCC 35221), and C. upsaliensis (LCDC 5424 [Laboratory Centre for Disease Control]).

Techniques: Isolation

Galectin-8 Is Expressed in Lymphoid Tissues (A) qRT-PCR analysis of Galectin-8 ( Lgals8 ) mRNA levels in kidney, bone marrow (BM), spleen, lymph nodes (LNs), and liver of C56BL/6 WT mice. Values were normalized with respect to the BM condition for each mouse. n = 5 mice pooled from N = 3 independent experiments. Bar graphs indicate mean ± SEM. (B) Representative images of H&E staining (left) and β-galactosidase staining (right) of LN cryosection from heterozygous mouse bearing a LacZ expression cassette on one allele of the Lgals8 locus. Arrowheads on the inset highlight β-galactosidase staining within the SCS area. Scale bar, 150 μm. (C) Representative images of serial lymph node cryosections stained for β-galactosidase (Galectin-8) and macrophages (Mac1) or B cells (B220). Scale bar, 200 μm. Zooms highlight the spatial localization of Galectin-8 together with macrophages and B cells at the SCS. Scale bar, 30 μm. See also <xref ref-type=Figure S1 . " width="100%" height="100%">

Journal: Cell Reports

Article Title: Galectin-8 Favors the Presentation of Surface-Tethered Antigens by Stabilizing the B Cell Immune Synapse

doi: 10.1016/j.celrep.2018.11.052

Figure Lengend Snippet: Galectin-8 Is Expressed in Lymphoid Tissues (A) qRT-PCR analysis of Galectin-8 ( Lgals8 ) mRNA levels in kidney, bone marrow (BM), spleen, lymph nodes (LNs), and liver of C56BL/6 WT mice. Values were normalized with respect to the BM condition for each mouse. n = 5 mice pooled from N = 3 independent experiments. Bar graphs indicate mean ± SEM. (B) Representative images of H&E staining (left) and β-galactosidase staining (right) of LN cryosection from heterozygous mouse bearing a LacZ expression cassette on one allele of the Lgals8 locus. Arrowheads on the inset highlight β-galactosidase staining within the SCS area. Scale bar, 150 μm. (C) Representative images of serial lymph node cryosections stained for β-galactosidase (Galectin-8) and macrophages (Mac1) or B cells (B220). Scale bar, 200 μm. Zooms highlight the spatial localization of Galectin-8 together with macrophages and B cells at the SCS. Scale bar, 30 μm. See also Figure S1 .

Article Snippet: Galectin-8 (primer: Mm01332239-m1 Lgals 8, Life Technologies) mRNA levels were assessed using TaqMan Gene Expression Master Mix (Applied Biosystems) according to manufacturer’s recommendations.

Techniques: Quantitative RT-PCR, Staining, Expressing

Galectin-8 Promotes B Cell Arrest Phases In Vivo (A) Representative western blot and quantification of the amounts of antigen (HEL) effectively immobilized at the surface of 0.2-μm microspheres in the presence of Galectin-8 or not. Data are pooled from N = 3 independent experiments. Bar graphs indicate mean ± SEM. (B) Schematic and representative image of the observed area within draining popliteal lymph nodes (pLN). Scale bar, 20 μm. The orthogonal view shows the deposit of microspheres at the periphery of pLN. (C) Representative images of HEL-specific migrating B cells (green) within the sub-capsular area of lymph nodes upon immunization with indicated microspheres (red). Scale bar, 8 μm. (D) Relative tracks of HEL-specific (MD4) and non-specific (WT) B cells. (E) Track straightness of B cells shown in (C). Boxes extend from the 25th to 75th percentile, with a line at the median and whiskers extend from the 10th to the 90th percentile. (F) Mean cell velocity of HEL-specific (gray) and non-specific (white) B cells upon immunization with indicated microspheres. Boxes extend from the 25th to 75th percentile, with a line at the median and whiskers extend from the 10th to the 90th percentile. (G) Quantification of the percentage of time cells spent at high (>2.5 μm/min; white), intermediate (between 1 and 2 μm/min; gray), and very slow (<1 μm/min; black) speed. (H) Mean cell velocity of HEL-specific B cells upon immunization with microspheres coated with non-relevant antigen (BSA) in combination or not with Galectin-8. Boxes extend from the 25th to 75th percentile, with a line at the median and whiskers extend from the 10th to the 90th percentile. In (D)–(H), n > 80 cells from 4 mice per condition pooled from N = 4 independent experiments. Kruskal-Wallis test was used to assess statistical significance. See also .

Journal: Cell Reports

Article Title: Galectin-8 Favors the Presentation of Surface-Tethered Antigens by Stabilizing the B Cell Immune Synapse

doi: 10.1016/j.celrep.2018.11.052

Figure Lengend Snippet: Galectin-8 Promotes B Cell Arrest Phases In Vivo (A) Representative western blot and quantification of the amounts of antigen (HEL) effectively immobilized at the surface of 0.2-μm microspheres in the presence of Galectin-8 or not. Data are pooled from N = 3 independent experiments. Bar graphs indicate mean ± SEM. (B) Schematic and representative image of the observed area within draining popliteal lymph nodes (pLN). Scale bar, 20 μm. The orthogonal view shows the deposit of microspheres at the periphery of pLN. (C) Representative images of HEL-specific migrating B cells (green) within the sub-capsular area of lymph nodes upon immunization with indicated microspheres (red). Scale bar, 8 μm. (D) Relative tracks of HEL-specific (MD4) and non-specific (WT) B cells. (E) Track straightness of B cells shown in (C). Boxes extend from the 25th to 75th percentile, with a line at the median and whiskers extend from the 10th to the 90th percentile. (F) Mean cell velocity of HEL-specific (gray) and non-specific (white) B cells upon immunization with indicated microspheres. Boxes extend from the 25th to 75th percentile, with a line at the median and whiskers extend from the 10th to the 90th percentile. (G) Quantification of the percentage of time cells spent at high (>2.5 μm/min; white), intermediate (between 1 and 2 μm/min; gray), and very slow (<1 μm/min; black) speed. (H) Mean cell velocity of HEL-specific B cells upon immunization with microspheres coated with non-relevant antigen (BSA) in combination or not with Galectin-8. Boxes extend from the 25th to 75th percentile, with a line at the median and whiskers extend from the 10th to the 90th percentile. In (D)–(H), n > 80 cells from 4 mice per condition pooled from N = 4 independent experiments. Kruskal-Wallis test was used to assess statistical significance. See also .

Article Snippet: Galectin-8 (primer: Mm01332239-m1 Lgals 8, Life Technologies) mRNA levels were assessed using TaqMan Gene Expression Master Mix (Applied Biosystems) according to manufacturer’s recommendations.

Techniques: In Vivo, Western Blot

Galectin-8 Promotes Particulate-Antigen Acquisition In Vivo (A) Representative image of popliteal lymph node cryosection from recipient mice, showing the distribution of HEL-coated (in absence of Galectin-8) microspheres (red) within the sub-capsular area and adoptively transferred Ag-specific B cells (green). Scale bar, 150 μm. (B) Quantification of the percentage of HEL-specific B cells loaded with HEL-coated microspheres in combination or not with Galectin-8. n > 100 cells pooled from N = 2 mice per condition. Unpaired t test was used to assess statistical significance. Bar graphs indicate mean ± SEM. (C) Representative images showing the internalization and accumulation of Ag-coated microspheres within Ag-specific B cells. Scale bar, 8 μm. (D) Distribution of the number of Ag-coated microspheres per B cells analyzed in (B).

Journal: Cell Reports

Article Title: Galectin-8 Favors the Presentation of Surface-Tethered Antigens by Stabilizing the B Cell Immune Synapse

doi: 10.1016/j.celrep.2018.11.052

Figure Lengend Snippet: Galectin-8 Promotes Particulate-Antigen Acquisition In Vivo (A) Representative image of popliteal lymph node cryosection from recipient mice, showing the distribution of HEL-coated (in absence of Galectin-8) microspheres (red) within the sub-capsular area and adoptively transferred Ag-specific B cells (green). Scale bar, 150 μm. (B) Quantification of the percentage of HEL-specific B cells loaded with HEL-coated microspheres in combination or not with Galectin-8. n > 100 cells pooled from N = 2 mice per condition. Unpaired t test was used to assess statistical significance. Bar graphs indicate mean ± SEM. (C) Representative images showing the internalization and accumulation of Ag-coated microspheres within Ag-specific B cells. Scale bar, 8 μm. (D) Distribution of the number of Ag-coated microspheres per B cells analyzed in (B).

Article Snippet: Galectin-8 (primer: Mm01332239-m1 Lgals 8, Life Technologies) mRNA levels were assessed using TaqMan Gene Expression Master Mix (Applied Biosystems) according to manufacturer’s recommendations.

Techniques: In Vivo

Exogenous Galectin-8 Favors T-B Cooperation In Vivo (A) Schematic of the experimental approach used to assess the ability of B cells to present antigen to CD4 + T cells in vivo . The western blot shows the amounts of antigen (HEL) effectively immobilized at the surface of 0.2-μm microspheres in the different conditions used to immunize mice. H, HEL alone; H-B, HEL-BSA; H-G, HEL-Galectin-8; H-B-O, HEL-BSA-OVA; H-G-O, HEL-Galectin-8-OVA. Numbers below the blot represent the normalized density of the bands with respect to the HEL alone condition. Data are representative of N = 3 independent experiments. (B and C) Representative dot plots used for the gating of (B) HEL-specific GC B cells (CD45.2 + B220 + HEL + GL7 + FAS + ) and (C) OVA-specific OT-II Tfh cells (CD45.2 + CD4 + CXCR5 + PD1 + ). (D and E) Quantification of the number of (D) HEL-specific GC B cells and (E) OVA-specific OT-II Tfh cells following mouse immunization with indicated microspheres. n = 10 mice per condition pooled from N = 3 independent experiments. (F and G) Quantification of the number of (F) HEL-specific GC B cells and (G) OVA-specific OT-II Tfh cells in CD45.1 Lgals8 +/+ or Lgals8 −/− recipient mice (adoptively transferred with CD45.2 Lgals8 +/+ SW HEL B cells and OT-II CD4 + T cells) following their immunization with indicated microspheres. n = 6 mice per condition pooled from N = 2 independent experiments. Kruskal-Wallis test was used to assess statistical significance. All bar graphs indicate mean ± SEM.

Journal: Cell Reports

Article Title: Galectin-8 Favors the Presentation of Surface-Tethered Antigens by Stabilizing the B Cell Immune Synapse

doi: 10.1016/j.celrep.2018.11.052

Figure Lengend Snippet: Exogenous Galectin-8 Favors T-B Cooperation In Vivo (A) Schematic of the experimental approach used to assess the ability of B cells to present antigen to CD4 + T cells in vivo . The western blot shows the amounts of antigen (HEL) effectively immobilized at the surface of 0.2-μm microspheres in the different conditions used to immunize mice. H, HEL alone; H-B, HEL-BSA; H-G, HEL-Galectin-8; H-B-O, HEL-BSA-OVA; H-G-O, HEL-Galectin-8-OVA. Numbers below the blot represent the normalized density of the bands with respect to the HEL alone condition. Data are representative of N = 3 independent experiments. (B and C) Representative dot plots used for the gating of (B) HEL-specific GC B cells (CD45.2 + B220 + HEL + GL7 + FAS + ) and (C) OVA-specific OT-II Tfh cells (CD45.2 + CD4 + CXCR5 + PD1 + ). (D and E) Quantification of the number of (D) HEL-specific GC B cells and (E) OVA-specific OT-II Tfh cells following mouse immunization with indicated microspheres. n = 10 mice per condition pooled from N = 3 independent experiments. (F and G) Quantification of the number of (F) HEL-specific GC B cells and (G) OVA-specific OT-II Tfh cells in CD45.1 Lgals8 +/+ or Lgals8 −/− recipient mice (adoptively transferred with CD45.2 Lgals8 +/+ SW HEL B cells and OT-II CD4 + T cells) following their immunization with indicated microspheres. n = 6 mice per condition pooled from N = 2 independent experiments. Kruskal-Wallis test was used to assess statistical significance. All bar graphs indicate mean ± SEM.

Article Snippet: Galectin-8 (primer: Mm01332239-m1 Lgals 8, Life Technologies) mRNA levels were assessed using TaqMan Gene Expression Master Mix (Applied Biosystems) according to manufacturer’s recommendations.

Techniques: In Vivo, Western Blot

Extracellular Galectin-8 Favors Lysosome Secretion at the B Cell Synapse (A) Quantification of the percentage of antigen (OVA) extracted from beads following incubation of primary spleen B cells with indicated beads and time. Values were normalized with respect to Ag-coated beads not engaged with B cells. n > 60 cells pooled from N = 2 independent experiments. Unpaired t test was used to assess statistical significance. Bar graphs indicate mean ± SEM. (B) Antigen ( Lack ) presentation assay with spleen B cells in presence or not of exogenous Galectin-8. Bars show the mean ± SEM of triplicate and are representative of N = 2 independent experiments. Unpaired t test was used to assess statistical significance. (C) Peptide controls for cells used in the antigen presentation assays shown in (B). Graph shows the mean of duplicates and is representative of N = 2 independent experiments. (D) Representative images of non-polarized and polarized centrosomes (γ-Tubulin) in primary B cells (left) and a B lymphoma cell line (right). Scale bar, 3 μm. The lower panel shows the quantification of the percentage of cells having polarized their centrosome following 60-min stimulation with BCR-ligand + beads containing or not Galectin-8. Paired t test was used to assess statistical significance. Bar graphs indicate mean ± SEM. n > 50 cell/bead conjugates assessed per condition pooled from N = 2 independent experiments (primary B cells) and N = 3 independent experiments (B cell line). (E) Representative images of B cells harboring the characteristic Lamp-1 + ring of lysosomes docked at the cell-bead interface. Scale bar, 3 μm. The lower panel shows the quantification of the percentage of cells displaying Lamp-1 + rings upon stimulation with BCR-ligand + beads containing or not Galectin-8 and in presence or not of 100 mM lactose for 60 min. n > 60 cell/bead conjugates assessed per condition pooled from N = 3 independent experiments. Kruskal-Wallis test was used to assess statistical significance. Bar graphs indicate mean ± SEM. (F) Representative images of B cells harboring acidic synapse as revealed by the appearance of Cypher5E fluorescence on the BCR-ligand + bead. Scale bar, 3 μm. The lower panel shows the quantification of the Cypher5E fluorescence intensity in cell-bead conjugates following a 90-min stimulation with BCR-ligand + beads containing or not Galectin-8. Values were normalized with respect to the mean fluorescence intensity of beads that were not engaged in immune synapses. n > 270 cell/bead conjugates assessed per condition pooled from N = 2 independent experiments. See also , , and .

Journal: Cell Reports

Article Title: Galectin-8 Favors the Presentation of Surface-Tethered Antigens by Stabilizing the B Cell Immune Synapse

doi: 10.1016/j.celrep.2018.11.052

Figure Lengend Snippet: Extracellular Galectin-8 Favors Lysosome Secretion at the B Cell Synapse (A) Quantification of the percentage of antigen (OVA) extracted from beads following incubation of primary spleen B cells with indicated beads and time. Values were normalized with respect to Ag-coated beads not engaged with B cells. n > 60 cells pooled from N = 2 independent experiments. Unpaired t test was used to assess statistical significance. Bar graphs indicate mean ± SEM. (B) Antigen ( Lack ) presentation assay with spleen B cells in presence or not of exogenous Galectin-8. Bars show the mean ± SEM of triplicate and are representative of N = 2 independent experiments. Unpaired t test was used to assess statistical significance. (C) Peptide controls for cells used in the antigen presentation assays shown in (B). Graph shows the mean of duplicates and is representative of N = 2 independent experiments. (D) Representative images of non-polarized and polarized centrosomes (γ-Tubulin) in primary B cells (left) and a B lymphoma cell line (right). Scale bar, 3 μm. The lower panel shows the quantification of the percentage of cells having polarized their centrosome following 60-min stimulation with BCR-ligand + beads containing or not Galectin-8. Paired t test was used to assess statistical significance. Bar graphs indicate mean ± SEM. n > 50 cell/bead conjugates assessed per condition pooled from N = 2 independent experiments (primary B cells) and N = 3 independent experiments (B cell line). (E) Representative images of B cells harboring the characteristic Lamp-1 + ring of lysosomes docked at the cell-bead interface. Scale bar, 3 μm. The lower panel shows the quantification of the percentage of cells displaying Lamp-1 + rings upon stimulation with BCR-ligand + beads containing or not Galectin-8 and in presence or not of 100 mM lactose for 60 min. n > 60 cell/bead conjugates assessed per condition pooled from N = 3 independent experiments. Kruskal-Wallis test was used to assess statistical significance. Bar graphs indicate mean ± SEM. (F) Representative images of B cells harboring acidic synapse as revealed by the appearance of Cypher5E fluorescence on the BCR-ligand + bead. Scale bar, 3 μm. The lower panel shows the quantification of the Cypher5E fluorescence intensity in cell-bead conjugates following a 90-min stimulation with BCR-ligand + beads containing or not Galectin-8. Values were normalized with respect to the mean fluorescence intensity of beads that were not engaged in immune synapses. n > 270 cell/bead conjugates assessed per condition pooled from N = 2 independent experiments. See also , , and .

Article Snippet: Galectin-8 (primer: Mm01332239-m1 Lgals 8, Life Technologies) mRNA levels were assessed using TaqMan Gene Expression Master Mix (Applied Biosystems) according to manufacturer’s recommendations.

Techniques: Incubation, Immunopeptidomics, Fluorescence

Galectin-8 Interacts with the BCR (A) GST-Galectin-8 pull-down experiments highlighting the absence of interaction between Galectin-8 and LFA-1 (lanes 1 and 2). Lane 3 shows the detection of LFA-1 in the whole-cell lysate (WCL). Representative of N = 4 independent experiments. (B) Quantification of the spreading area of B cells pre-treated or not for 30 min with 10 μg/mL function-blocking anti-LFA-1 antibody and seeded on poly- l -lysine (PLL)- or Galectin-8-coated coverslips. n > 60 cells per condition pooled from N = 3 independent experiments. ANOVA test followed by a Sidak’s multiple-comparison test was used to assess statistical significance. Bar graphs indicate mean ± SEM. (C) Flow cytometry quantification of cell-bound Alexa Fluor 488-conjugated Galectin-8 following a 60-min incubation with B cells pre-treated or not for 30 min with 2 μg/mL recombinant ICAM-1 or 10 μg/mL function-blocking anti-LFA-1 antibody. Values were normalized with respect to the untreated condition in each experimental replicate. Data are pooled from N = 2 independent experiments. ANOVA test followed by a Sidak’s multiple-comparison test was used to assess statistical significance. Bar graphs indicate mean ± SEM. (D) GST-Galectin-8 pulls down the BCR from B cell extracts (lane 1), which is inhibited in presence of 100 mM lactose (lane 2), indicating the dependency of Galectin-8/glycan interactions. Lane 3 shows the detection of the BCR in the whole-cell lysate (WCL). ∗ The higher molecular weight could correspond to a highly glycosylated form of the BCR or a stable complex between the Ig heavy and light chain. Representative of N = 3 independent experiments. (E) Flow cytometry analysis of the phosphorylation status of BCR-downstream signaling molecules upon stimulation of primary spleen B cells with BCR-ligand + beads containing or not Galectin-8 for indicated times. Values were normalized with respect to the non-stimulated condition for each mouse. Each dot on the bar graphs corresponds to cells purified from independent mice. Data are representative of N = 2 independent experiments. Ratio t test was used to assess statistical significance. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗∗ p < 0.0001. Bar graphs indicate mean ± SEM. See also .

Journal: Cell Reports

Article Title: Galectin-8 Favors the Presentation of Surface-Tethered Antigens by Stabilizing the B Cell Immune Synapse

doi: 10.1016/j.celrep.2018.11.052

Figure Lengend Snippet: Galectin-8 Interacts with the BCR (A) GST-Galectin-8 pull-down experiments highlighting the absence of interaction between Galectin-8 and LFA-1 (lanes 1 and 2). Lane 3 shows the detection of LFA-1 in the whole-cell lysate (WCL). Representative of N = 4 independent experiments. (B) Quantification of the spreading area of B cells pre-treated or not for 30 min with 10 μg/mL function-blocking anti-LFA-1 antibody and seeded on poly- l -lysine (PLL)- or Galectin-8-coated coverslips. n > 60 cells per condition pooled from N = 3 independent experiments. ANOVA test followed by a Sidak’s multiple-comparison test was used to assess statistical significance. Bar graphs indicate mean ± SEM. (C) Flow cytometry quantification of cell-bound Alexa Fluor 488-conjugated Galectin-8 following a 60-min incubation with B cells pre-treated or not for 30 min with 2 μg/mL recombinant ICAM-1 or 10 μg/mL function-blocking anti-LFA-1 antibody. Values were normalized with respect to the untreated condition in each experimental replicate. Data are pooled from N = 2 independent experiments. ANOVA test followed by a Sidak’s multiple-comparison test was used to assess statistical significance. Bar graphs indicate mean ± SEM. (D) GST-Galectin-8 pulls down the BCR from B cell extracts (lane 1), which is inhibited in presence of 100 mM lactose (lane 2), indicating the dependency of Galectin-8/glycan interactions. Lane 3 shows the detection of the BCR in the whole-cell lysate (WCL). ∗ The higher molecular weight could correspond to a highly glycosylated form of the BCR or a stable complex between the Ig heavy and light chain. Representative of N = 3 independent experiments. (E) Flow cytometry analysis of the phosphorylation status of BCR-downstream signaling molecules upon stimulation of primary spleen B cells with BCR-ligand + beads containing or not Galectin-8 for indicated times. Values were normalized with respect to the non-stimulated condition for each mouse. Each dot on the bar graphs corresponds to cells purified from independent mice. Data are representative of N = 2 independent experiments. Ratio t test was used to assess statistical significance. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗∗ p < 0.0001. Bar graphs indicate mean ± SEM. See also .

Article Snippet: Galectin-8 (primer: Mm01332239-m1 Lgals 8, Life Technologies) mRNA levels were assessed using TaqMan Gene Expression Master Mix (Applied Biosystems) according to manufacturer’s recommendations.

Techniques: Blocking Assay, Comparison, Flow Cytometry, Incubation, Recombinant, Glycoproteomics, Molecular Weight, Phospho-proteomics, Purification

Journal: Cell Reports

Article Title: Galectin-8 Favors the Presentation of Surface-Tethered Antigens by Stabilizing the B Cell Immune Synapse

doi: 10.1016/j.celrep.2018.11.052

Figure Lengend Snippet:

Article Snippet: Galectin-8 (primer: Mm01332239-m1 Lgals 8, Life Technologies) mRNA levels were assessed using TaqMan Gene Expression Master Mix (Applied Biosystems) according to manufacturer’s recommendations.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Sequencing, Software

Bacterial strains and results of PCR assays in which species-specific primers BiLON, BiINF, BiDEN, and BiGAL were used a

Journal:

Article Title: Distribution of Bifidobacterial Species in Human Intestinal Microflora Examined with 16S rRNA-Gene-Targeted Species-Specific Primers

doi:

Figure Lengend Snippet: Bacterial strains and results of PCR assays in which species-specific primers BiLON, BiINF, BiDEN, and BiGAL were used a

Article Snippet: Six edge fields and four center fields were counted, and the counts were then correlated with the actual sample size ( 9 ). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Species Strain(s) b Species-specific primer PCR results c BiLON BiINF BiDEN BiGAL B. adolescentis ATCC 15703 T , NCFB 2229, NCFB 2230, NCFB 2231 − − − − B. angulatum ATCC 27535 T , JCM 1252 − − − − B. bifidum ATCC 29521 T , ATCC 15696, ATCC 11863, Yakult d − − − − B. breve ATCC 15700 T , ATCC 15698, ATCC 15701, Yakult d − − − − B. catenulatum ATCC 27539 T , JCM 7130 − − − − B. pseudocatenulatum JCM 1200 T , DSM 20439 − − − − B. longum ATCC 15707 T , ATCC 15708, FERM P-6548 + − − − B. infantis ATCC 15697 T , ATCC 15702, ATCC 25962 − + − − B. suis ATCC 27533 T + − − − B. dentium ATCC 27534 T , DSM 20084, DSM 20221 − − + − B. gallicum JCM 8224 T − − − + Open in a separate window a In addition to the bacteria listed, negative PCR results with primers BiLON, BiINF, BiDEN, and BiGAL were obtained for the following bacterial species: B. animalis ATCC 25527 T , B. asteroides ATCC 25910 T , B. boum JCM 1211 T , B. choerinum ATCC 27686 T , B. coryneforme ATCC 25911 T , B. cuniculi ATCC 27916 T , B. denticolens DSM 10105 T , B. gallinarum JCM 6291 T , B. indicum ATCC 25912 T , B. inopinatum DSM 10107 T , B. lactis DSM 10140 T , B. magnum JCM 1218 T , B. merycicum JCM 8219 T , B. minimum ATCC 27538 T , B. pseudolongum subsp. globosum ATCC 25864 T , B. pseudolongum subsp. pseudolongum JCM 1205 T , B. pullorum JCM 1214 T , B. ruminantium JCM 8222 T , B. saeculare DSM 6531 T , B. subtile DSM 20096 T , B. thermophilium ATCC 25866 T , E. coli ATCC 11775 T , Bacteroides fragilis NCTC 9343 T , Bacteroides ovatus JCM 5824 T , Bacteroides vulgatus ATCC 8424 T , Clostridium bifermentans JCM 1386 T , Clostridium perfringens JCM 1290 T , Enterococcus faecalis ATCC 19433 T , Enterococcus faecium ATCC 19434 T , Eubacterium aerofaciens ATCC 25986 T , Eubacterium biforme ATCC 27806 T , Gardnerella vaginalis DSM 4944 T , Lactobacillus acidophilus ATCC 4356 T , Propionibacterium acnes ATCC 6919 T , Peptostreptococcus prevotii ATCC 9321 T , and Ruminococcus productus ATCC 27340 T . b ATCC, American Type Culture Collection; JCM, Japan Collection of Microorganisms; DSM, German Collection of Microorganisms and Cell Cultures; NCFB, National Collection of Food Bacteria; NCTC, National Collection of Type Cultures; FERM, National Institute of Biosciences and Human Technology. c The PCR specificities of other species-specific primers, such as BiADO, BiANG, BiBIF, BiBRE, and BiCATg, have been reported previously ( 16 ). d These strains are used in a probiotic culture in dairy products and were obtained from Yakult Central Institute for Microbiological Research.

Techniques:

Bifidobacterium species- and group-specific primers based on 16S rDNA sequences

Journal:

Article Title: Distribution of Bifidobacterial Species in Human Intestinal Microflora Examined with 16S rRNA-Gene-Targeted Species-Specific Primers

doi:

Figure Lengend Snippet: Bifidobacterium species- and group-specific primers based on 16S rDNA sequences

Article Snippet: Six edge fields and four center fields were counted, and the counts were then correlated with the actual sample size ( 9 ). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Species Strain(s) b Species-specific primer PCR results c BiLON BiINF BiDEN BiGAL B. adolescentis ATCC 15703 T , NCFB 2229, NCFB 2230, NCFB 2231 − − − − B. angulatum ATCC 27535 T , JCM 1252 − − − − B. bifidum ATCC 29521 T , ATCC 15696, ATCC 11863, Yakult d − − − − B. breve ATCC 15700 T , ATCC 15698, ATCC 15701, Yakult d − − − − B. catenulatum ATCC 27539 T , JCM 7130 − − − − B. pseudocatenulatum JCM 1200 T , DSM 20439 − − − − B. longum ATCC 15707 T , ATCC 15708, FERM P-6548 + − − − B. infantis ATCC 15697 T , ATCC 15702, ATCC 25962 − + − − B. suis ATCC 27533 T + − − − B. dentium ATCC 27534 T , DSM 20084, DSM 20221 − − + − B. gallicum JCM 8224 T − − − + Open in a separate window a In addition to the bacteria listed, negative PCR results with primers BiLON, BiINF, BiDEN, and BiGAL were obtained for the following bacterial species: B. animalis ATCC 25527 T , B. asteroides ATCC 25910 T , B. boum JCM 1211 T , B. choerinum ATCC 27686 T , B. coryneforme ATCC 25911 T , B. cuniculi ATCC 27916 T , B. denticolens DSM 10105 T , B. gallinarum JCM 6291 T , B. indicum ATCC 25912 T , B. inopinatum DSM 10107 T , B. lactis DSM 10140 T , B. magnum JCM 1218 T , B. merycicum JCM 8219 T , B. minimum ATCC 27538 T , B. pseudolongum subsp. globosum ATCC 25864 T , B. pseudolongum subsp. pseudolongum JCM 1205 T , B. pullorum JCM 1214 T , B. ruminantium JCM 8222 T , B. saeculare DSM 6531 T , B. subtile DSM 20096 T , B. thermophilium ATCC 25866 T , E. coli ATCC 11775 T , Bacteroides fragilis NCTC 9343 T , Bacteroides ovatus JCM 5824 T , Bacteroides vulgatus ATCC 8424 T , Clostridium bifermentans JCM 1386 T , Clostridium perfringens JCM 1290 T , Enterococcus faecalis ATCC 19433 T , Enterococcus faecium ATCC 19434 T , Eubacterium aerofaciens ATCC 25986 T , Eubacterium biforme ATCC 27806 T , Gardnerella vaginalis DSM 4944 T , Lactobacillus acidophilus ATCC 4356 T , Propionibacterium acnes ATCC 6919 T , Peptostreptococcus prevotii ATCC 9321 T , and Ruminococcus productus ATCC 27340 T . b ATCC, American Type Culture Collection; JCM, Japan Collection of Microorganisms; DSM, German Collection of Microorganisms and Cell Cultures; NCFB, National Collection of Food Bacteria; NCTC, National Collection of Type Cultures; FERM, National Institute of Biosciences and Human Technology. c The PCR specificities of other species-specific primers, such as BiADO, BiANG, BiBIF, BiBRE, and BiCATg, have been reported previously ( 16 ). d These strains are used in a probiotic culture in dairy products and were obtained from Yakult Central Institute for Microbiological Research.

Techniques: Sequencing

PCR products obtained for 10 Bifidobacterium species with their specific primers. Lane M, DNA size markers (sizes [in bases] are indicated on the left); lane 1, B. adolescentis ATCC 15703T; lane 2, B. angulatum ATCC 27535T; lane 3, B. bifidum ATCC 29521T; lane 4, B. breve ATCC 15700T; lane 5, B. catenulatum ATCC 27539T; lane 6, B. pseudocatenulatum JCM 1200T; lane 7, B. longum ATCC 157071T; lane 8, B. infantis ATCC 15697T; lane 9, B. dentium ATCC 27534T; lane 10, B. gallicum JCM 8224T; lane 11, negative control (PCR performed with primer BiADO and E. coli ATCC 11775T).

Journal:

Article Title: Distribution of Bifidobacterial Species in Human Intestinal Microflora Examined with 16S rRNA-Gene-Targeted Species-Specific Primers

doi:

Figure Lengend Snippet: PCR products obtained for 10 Bifidobacterium species with their specific primers. Lane M, DNA size markers (sizes [in bases] are indicated on the left); lane 1, B. adolescentis ATCC 15703T; lane 2, B. angulatum ATCC 27535T; lane 3, B. bifidum ATCC 29521T; lane 4, B. breve ATCC 15700T; lane 5, B. catenulatum ATCC 27539T; lane 6, B. pseudocatenulatum JCM 1200T; lane 7, B. longum ATCC 157071T; lane 8, B. infantis ATCC 15697T; lane 9, B. dentium ATCC 27534T; lane 10, B. gallicum JCM 8224T; lane 11, negative control (PCR performed with primer BiADO and E. coli ATCC 11775T).

Article Snippet: Six edge fields and four center fields were counted, and the counts were then correlated with the actual sample size ( 9 ). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Species Strain(s) b Species-specific primer PCR results c BiLON BiINF BiDEN BiGAL B. adolescentis ATCC 15703 T , NCFB 2229, NCFB 2230, NCFB 2231 − − − − B. angulatum ATCC 27535 T , JCM 1252 − − − − B. bifidum ATCC 29521 T , ATCC 15696, ATCC 11863, Yakult d − − − − B. breve ATCC 15700 T , ATCC 15698, ATCC 15701, Yakult d − − − − B. catenulatum ATCC 27539 T , JCM 7130 − − − − B. pseudocatenulatum JCM 1200 T , DSM 20439 − − − − B. longum ATCC 15707 T , ATCC 15708, FERM P-6548 + − − − B. infantis ATCC 15697 T , ATCC 15702, ATCC 25962 − + − − B. suis ATCC 27533 T + − − − B. dentium ATCC 27534 T , DSM 20084, DSM 20221 − − + − B. gallicum JCM 8224 T − − − + Open in a separate window a In addition to the bacteria listed, negative PCR results with primers BiLON, BiINF, BiDEN, and BiGAL were obtained for the following bacterial species: B. animalis ATCC 25527 T , B. asteroides ATCC 25910 T , B. boum JCM 1211 T , B. choerinum ATCC 27686 T , B. coryneforme ATCC 25911 T , B. cuniculi ATCC 27916 T , B. denticolens DSM 10105 T , B. gallinarum JCM 6291 T , B. indicum ATCC 25912 T , B. inopinatum DSM 10107 T , B. lactis DSM 10140 T , B. magnum JCM 1218 T , B. merycicum JCM 8219 T , B. minimum ATCC 27538 T , B. pseudolongum subsp. globosum ATCC 25864 T , B. pseudolongum subsp. pseudolongum JCM 1205 T , B. pullorum JCM 1214 T , B. ruminantium JCM 8222 T , B. saeculare DSM 6531 T , B. subtile DSM 20096 T , B. thermophilium ATCC 25866 T , E. coli ATCC 11775 T , Bacteroides fragilis NCTC 9343 T , Bacteroides ovatus JCM 5824 T , Bacteroides vulgatus ATCC 8424 T , Clostridium bifermentans JCM 1386 T , Clostridium perfringens JCM 1290 T , Enterococcus faecalis ATCC 19433 T , Enterococcus faecium ATCC 19434 T , Eubacterium aerofaciens ATCC 25986 T , Eubacterium biforme ATCC 27806 T , Gardnerella vaginalis DSM 4944 T , Lactobacillus acidophilus ATCC 4356 T , Propionibacterium acnes ATCC 6919 T , Peptostreptococcus prevotii ATCC 9321 T , and Ruminococcus productus ATCC 27340 T . b ATCC, American Type Culture Collection; JCM, Japan Collection of Microorganisms; DSM, German Collection of Microorganisms and Cell Cultures; NCFB, National Collection of Food Bacteria; NCTC, National Collection of Type Cultures; FERM, National Institute of Biosciences and Human Technology. c The PCR specificities of other species-specific primers, such as BiADO, BiANG, BiBIF, BiBRE, and BiCATg, have been reported previously ( 16 ). d These strains are used in a probiotic culture in dairy products and were obtained from Yakult Central Institute for Microbiological Research.

Techniques: Negative Control

Distribution of Bifidobacterium species in human adults and infants a

Journal:

Article Title: Distribution of Bifidobacterial Species in Human Intestinal Microflora Examined with 16S rRNA-Gene-Targeted Species-Specific Primers

doi:

Figure Lengend Snippet: Distribution of Bifidobacterium species in human adults and infants a

Article Snippet: Six edge fields and four center fields were counted, and the counts were then correlated with the actual sample size ( 9 ). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Species Strain(s) b Species-specific primer PCR results c BiLON BiINF BiDEN BiGAL B. adolescentis ATCC 15703 T , NCFB 2229, NCFB 2230, NCFB 2231 − − − − B. angulatum ATCC 27535 T , JCM 1252 − − − − B. bifidum ATCC 29521 T , ATCC 15696, ATCC 11863, Yakult d − − − − B. breve ATCC 15700 T , ATCC 15698, ATCC 15701, Yakult d − − − − B. catenulatum ATCC 27539 T , JCM 7130 − − − − B. pseudocatenulatum JCM 1200 T , DSM 20439 − − − − B. longum ATCC 15707 T , ATCC 15708, FERM P-6548 + − − − B. infantis ATCC 15697 T , ATCC 15702, ATCC 25962 − + − − B. suis ATCC 27533 T + − − − B. dentium ATCC 27534 T , DSM 20084, DSM 20221 − − + − B. gallicum JCM 8224 T − − − + Open in a separate window a In addition to the bacteria listed, negative PCR results with primers BiLON, BiINF, BiDEN, and BiGAL were obtained for the following bacterial species: B. animalis ATCC 25527 T , B. asteroides ATCC 25910 T , B. boum JCM 1211 T , B. choerinum ATCC 27686 T , B. coryneforme ATCC 25911 T , B. cuniculi ATCC 27916 T , B. denticolens DSM 10105 T , B. gallinarum JCM 6291 T , B. indicum ATCC 25912 T , B. inopinatum DSM 10107 T , B. lactis DSM 10140 T , B. magnum JCM 1218 T , B. merycicum JCM 8219 T , B. minimum ATCC 27538 T , B. pseudolongum subsp. globosum ATCC 25864 T , B. pseudolongum subsp. pseudolongum JCM 1205 T , B. pullorum JCM 1214 T , B. ruminantium JCM 8222 T , B. saeculare DSM 6531 T , B. subtile DSM 20096 T , B. thermophilium ATCC 25866 T , E. coli ATCC 11775 T , Bacteroides fragilis NCTC 9343 T , Bacteroides ovatus JCM 5824 T , Bacteroides vulgatus ATCC 8424 T , Clostridium bifermentans JCM 1386 T , Clostridium perfringens JCM 1290 T , Enterococcus faecalis ATCC 19433 T , Enterococcus faecium ATCC 19434 T , Eubacterium aerofaciens ATCC 25986 T , Eubacterium biforme ATCC 27806 T , Gardnerella vaginalis DSM 4944 T , Lactobacillus acidophilus ATCC 4356 T , Propionibacterium acnes ATCC 6919 T , Peptostreptococcus prevotii ATCC 9321 T , and Ruminococcus productus ATCC 27340 T . b ATCC, American Type Culture Collection; JCM, Japan Collection of Microorganisms; DSM, German Collection of Microorganisms and Cell Cultures; NCFB, National Collection of Food Bacteria; NCTC, National Collection of Type Cultures; FERM, National Institute of Biosciences and Human Technology. c The PCR specificities of other species-specific primers, such as BiADO, BiANG, BiBIF, BiBRE, and BiCATg, have been reported previously ( 16 ). d These strains are used in a probiotic culture in dairy products and were obtained from Yakult Central Institute for Microbiological Research.

Techniques:

Comparison of the species-specific primer method with the classical culture method when the same samples were used

Journal:

Article Title: Distribution of Bifidobacterial Species in Human Intestinal Microflora Examined with 16S rRNA-Gene-Targeted Species-Specific Primers

doi:

Figure Lengend Snippet: Comparison of the species-specific primer method with the classical culture method when the same samples were used

Article Snippet: Six edge fields and four center fields were counted, and the counts were then correlated with the actual sample size ( 9 ). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Species Strain(s) b Species-specific primer PCR results c BiLON BiINF BiDEN BiGAL B. adolescentis ATCC 15703 T , NCFB 2229, NCFB 2230, NCFB 2231 − − − − B. angulatum ATCC 27535 T , JCM 1252 − − − − B. bifidum ATCC 29521 T , ATCC 15696, ATCC 11863, Yakult d − − − − B. breve ATCC 15700 T , ATCC 15698, ATCC 15701, Yakult d − − − − B. catenulatum ATCC 27539 T , JCM 7130 − − − − B. pseudocatenulatum JCM 1200 T , DSM 20439 − − − − B. longum ATCC 15707 T , ATCC 15708, FERM P-6548 + − − − B. infantis ATCC 15697 T , ATCC 15702, ATCC 25962 − + − − B. suis ATCC 27533 T + − − − B. dentium ATCC 27534 T , DSM 20084, DSM 20221 − − + − B. gallicum JCM 8224 T − − − + Open in a separate window a In addition to the bacteria listed, negative PCR results with primers BiLON, BiINF, BiDEN, and BiGAL were obtained for the following bacterial species: B. animalis ATCC 25527 T , B. asteroides ATCC 25910 T , B. boum JCM 1211 T , B. choerinum ATCC 27686 T , B. coryneforme ATCC 25911 T , B. cuniculi ATCC 27916 T , B. denticolens DSM 10105 T , B. gallinarum JCM 6291 T , B. indicum ATCC 25912 T , B. inopinatum DSM 10107 T , B. lactis DSM 10140 T , B. magnum JCM 1218 T , B. merycicum JCM 8219 T , B. minimum ATCC 27538 T , B. pseudolongum subsp. globosum ATCC 25864 T , B. pseudolongum subsp. pseudolongum JCM 1205 T , B. pullorum JCM 1214 T , B. ruminantium JCM 8222 T , B. saeculare DSM 6531 T , B. subtile DSM 20096 T , B. thermophilium ATCC 25866 T , E. coli ATCC 11775 T , Bacteroides fragilis NCTC 9343 T , Bacteroides ovatus JCM 5824 T , Bacteroides vulgatus ATCC 8424 T , Clostridium bifermentans JCM 1386 T , Clostridium perfringens JCM 1290 T , Enterococcus faecalis ATCC 19433 T , Enterococcus faecium ATCC 19434 T , Eubacterium aerofaciens ATCC 25986 T , Eubacterium biforme ATCC 27806 T , Gardnerella vaginalis DSM 4944 T , Lactobacillus acidophilus ATCC 4356 T , Propionibacterium acnes ATCC 6919 T , Peptostreptococcus prevotii ATCC 9321 T , and Ruminococcus productus ATCC 27340 T . b ATCC, American Type Culture Collection; JCM, Japan Collection of Microorganisms; DSM, German Collection of Microorganisms and Cell Cultures; NCFB, National Collection of Food Bacteria; NCTC, National Collection of Type Cultures; FERM, National Institute of Biosciences and Human Technology. c The PCR specificities of other species-specific primers, such as BiADO, BiANG, BiBIF, BiBRE, and BiCATg, have been reported previously ( 16 ). d These strains are used in a probiotic culture in dairy products and were obtained from Yakult Central Institute for Microbiological Research.

Techniques: